Fig. 8. Adenovirus infection causes UBF to separate from RNA pol I and the major sites of RNA synthesis. (A) UBF (in green) and RNA synthesis (detected by FU labelling for 20 minutes as outlined in the methods and shown in red) in uninfected cells. (B) As A, but in adenovirus-infected cells at 18 hours post-infection. C shows the location of UBF (green) and RNA pol I (red) in uninfected cells, and D shows the same in adenovirus-infected cells at 18 hours post-infection. (E) Adenovirus-infected cells were FU labelled as before and simultaneously probed for RNA synthesis (green), RNA Pol I (red) and viral DBP (blue). (F) As for E, but probed for UBF (green), RNA pol I (red) and FU (blue). All images are of a single focal plane approximately 0.3 µm depth; bar, 10 µm.