Fig. 10. Co-expression of ßPIX and GIT1-derived constructs in retinal neurons. (A-I) Retinal neurons were fixed 1 day after transfection or cotransfection, and the indicated transfected constructs were detected by immunofluorescence. Bars, 5 µm. (J) Transfected neurons were utilized to quantify the effects on neurite extension. Each bar represents the average percentage obtained from two experiments for each condition; 50 neurons were analyzed in each experiments (total of 100 neurons per condition). (K) Evaluation of the percentages of neurons with long neurites and of neurons with large cytoplasmic structures in neurons transfected with the indicated constructs: 100 neurons per condition were evaluated.