Fig. 7. Effect of Y-27632 on HIF-1 expression in MSCs with or without CoCl₂ treatment. (A) HIF-1 immunostaining observed with confocal microscopy on MSCs treated with CoCl₂ and/or Y-27632 for 3 hours. Bar, 20 µm. (B) HIF-1 western blot performed from nuclear extracts after 3 hours of CoCl₂ and/or Y-27632 treatment. (C) Luciferase activity measurement after 3, 8, 24 and 36 hours of CoCl₂ and/or Y-27632 treatment. Firefly luciferase activities were normalized with the internal control Renilla luciferase activity. The fold induction represents the luciferase activity observed in transfection with the HRE-pGL3SV40 plasmid relative to the empty plasmid (pGL3SV40). The values are represented as fold induction compared with control ± s.e.m., n=3, *P<0.05, #P<0.0001, PLSD Fisher.