Fig. 8. ICP0 is sufficient to redistribute ATRIP in transfected cells. Vero cells were transfected with an expression plasmid encoding the ICP0 gene (pICP0) and fixed in 4% PFA as described in the Materials and Methods. (A) Merged image of a transfected cell double labeled with mouse anti-ICP0 (green) and rabbit anti-P-RPA (red). No colocalization of the two proteins was observed. (B) Merged image of a transfected cell double labeled with rat anti-Hsc70, a cellular marker that stains the interior of ICP0 inclusions (green), and mouse anti-ATRIP (red). Note the ATRIP-stained ICP0-like nuclear inclusions surrounding Hsc70. (C) Merged image of a transfected cell double labeled with mouse anti-ICP0 (green) and rabbit anti-ATR (red). No colocalization of the two proteins was observed. 100x magnification with 2x zoom.