Fig. 5. Localisation of 53BP1 after DNA damage. (A) Untreated HeLa cells or cells treated with cisplatin for 4 hours or UV irradiation with a recovery time of 0.5 hours were stained with antibody to 53BP1 and counterstained with DAPI. (B) HeLa cells transfected with the indicated GFP-tagged lamin A constructs (green) were treated with cisplatin for 4 hours, fixed and stained with antibody to 53BP1 (red) and counterstained with DAPI. Arrows indicate transfected cells showing diffuse 53BP1 staining. Bar, 10 µm. (C) Quantitative analysis of cells showing 53BP1 staining in nuclear foci after cisplatin treatment. Ut, untransfected.