Fig. 4. Rac1 affects podosome assembly. Wt, Rac1^–/–, Rac2^–/– and Rac1/2^–/– BMMs in macrophage growth medium were stained for F-actin (red in merged image) and paxillin (green in merged image). A higher magnification image of podosomes in shown in the top right hand corner of Wt and Rac1^–/– BMM images. Bars, 10 µm.