Fig. 6. Chs2p is required for proper CAR contraction and stability. (A,B) Cdc15-GFP was used to visualize the CAR in either a WT or chs2 cells using conventional (A) or confocal (B) microscopy. (A) The Hht2-GFP histone was used to compare the progression of the nuclear cycle and the images shown were taken every 7 minutes after anaphase completion. (B) Time-lapse images of WT and chs2 cells carrying Cdc15-GFP, taken every 3 minutes. Lateral and frontal views of the three-dimensional reconstructions are shown. Numbers indicate the order of the time-point series taken after the initiation of CAR contraction. Arrowheads point to abnormal structures detected in the Cdc15p ring in the chs2 strain. (C) cdc25-22 or cdc25-22 chs2 cultures were arrested at 37°C and released at 25°C. Samples were collected at the indicated times, stained with DAPI and Calcofluor, and scored for the presence of nuclei and complete septa.