Fig. 1. Disruption of the cell membrane induces disassembly and reassembly of MTs. (A) A PtK2 cell expressing
-tubulin-EGFP was wounded by a glass needle in 1.8 mM Ca2+ Ringer's solution. The asterisk indicates the site of cell membrane disruption. The membrane disruption initially induced MT disassembly around the wound site. Arrows indicate examples of disassembled MTs. (B,C) Plus-end tips of disassembled MTs approached the wound site in 1.8 mM Ca2+ Ringer's solution. Colored dots indicate selected MT ends.