Fig. 2. Comparative study for the detection of hTERT protein in HeLa cells by immunoblotting and immunoprecipitation using commercially available antibodies. (A) Western blot analysis of total cell lysates from HeLa cells transfected with either HA-tagged hTERT (pB-hTERT-HA) or p-Babe empty vector alone (pB) using an anti-HA antibody and various anti-hTERT antibodies. (B) Immunoprecipitation (IP) of lysates from HeLa cells transfected with HA-tagged hTERT (pB-hTERT-HA), the non-tagged hTERT protein (pB-hTERT), or p-Babe empty vector alone (pB) with anti-HA antibody and immunoblotting with either anti-HA antibody or various anti-hTERT antibodies as indicated. Positions of the molecular weight markers are indicated on the left. Positions of the proteins of interest are indicated by arrows. Position of the heavy chain (Ig) of the anti-HA antibody is shown.