Fig. 1. Localization of KIF17b and MIWI in the chromatoid body. (A,B) Squash preparations at stage VI (A), or drying down slides of male germ cells from stages II-VI (B) were immunostained with anti-KIF17b antibody (red). (C,D) Concentration of MIWI in chromatoid bodies as shown by immunostaining of squash preparation (C) or drying down slides (D) with anti-MIWI antibody (red). The parallel phase contrast image in panels B and D demonstrate the location of chromatoid bodies. Alexa Fluor 594 anti-rabbit IgG was used as a secondary antibody, and nuclei are stained blue with DAPI. Bar in A and C, 10 µm; bar in B and D, 5 µm.