Fig. 2. KIF17b interacts with MIWI. Interaction of the full-length KIF17b with MIWI. COS-1 cells were transfected with expression plasmids encoding Myc-tagged KIF17b and FLAG-MIWI. Immunoprecipitation (IP) was performed from the cell lysates with anti-FLAG antibody (
-FLAG), and the samples were immunoblotted either by anti-Myc antibody (
-Myc) to detect co-immunoprecipitated KIF17b or by anti-FLAG antibody to detect MIWI. A non-specific band crossreacting with the anti-FLAG antibody is indicated by an asterisk.