JCS -- Dovas et al. 119 (13): 2837 Figure IG1

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Fig. 1. Expression and intracellular localization of conventional and novel PKC isozymes in REF cells. (A) PKC isoforms expressed in REF cells. REF cell lysates were resolved by SDS-PAGE and probed with monoclonal antibodies against the PKC isoforms indicated. +ve, positive control; REF, REF cell lysate. (B) Differential localization of endogenous PKCs ${alpha}$ , ${delta}$ and ${epsilon}$ with respect to the actin cytoskeleton. PKC ${alpha}$ is localized at the ends of stress fibres (focal adhesions) and in a juxtanuclear pool, whereas PKC ${delta}$ is mostly cytoplasmic with some staining associated with cortical actin (arrows). PKC ${epsilon}$ is perinuclear and shows a Golgi-like staining. Bar, 50 µm.