JCS -- Dovas et al. 119 (13): 2837 Figure IG8

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Fig. 8. LPA depends on RhoA-ROCK, not PKC ${alpha}$ , in order to phosphorylate myosin light chain. REFs plated on FN110 for 1.5 hours were treated with Gö6976, Y-27632 or pre-treated with TAT-C3 before plating as indicated in the Materials and Methods, followed by stimulation with LPA at 2 hours. After a further 30 minutes, cells were fixed, permeabilized and stained for F-actin and phospho-MLC (pMLC2). Bar, 50 µm.