Fig. 3. Organization of actin cables is not affected in sec12 cells. The wild-type (SEY6210) and sec12 (FBY217) strains grown at 24°C were fixed, permeabilized and incubated with Texas Red-phalloidin (TR-phalloidin) as described in Materials and Methods before collecting fluorescence images. The sec12 mutant was also treated and imaged as above after being incubated at restrictive temperature for 90 minutes. DIC, differential-interference-contrast images.