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Figure 4


Fig. 4. Atg9 sorting from mitochondria is impaired in the absence of the VFT complex. The wild-type (SEY6210) and vps52{Delta} (PSY113) strains both transformed with the plasmid expressing Spo7-GFP (YEplac122-TRP1-SPO7-GFP) were grown at 30°C to an early log-phase in rich medium (A) or starved of nitrogen for 2 hours (B). Cells were labeled with MitoFluor Red and imaged as described in Fig. 1. The mitochondrial reticulum was disrupted in the absence of VPS52 in rich medium. DIC, differential-interference-contrast images.