Fig. 5. Alteration of subcellular structures in the vps52 mutant. (A) Mitochondrial morphology is severely compromised in the absence of Vps52. The vps52 (PSY113) strain was grown in rich medium to an early log-phase and mitochondria labeled by adding MitoFluor Red prior to imaging. (B) Actin cables are aberrantly organized in the vps52 mutant. Cells grown as in panel A were fixed, permeabilized and incubated with Texas Red-phalloidin (TR-phalloidin) as described in Materials and Methods before collecting fluorescence images. DIC, differential-interference-contrast images.