Fig. 7. Atg9 trafficking from mitochondria is not affected in the absence of Vps4. The vps4 Atg9-YFP (FRY304) and vps4 atg1 Atg9-YFP (FRY305) strains were grown in rich medium, subjected to mild fixation and then photographed. Vacuoles were labeled by adding FM 4-64 FX 1 hour prior to imaging. The YFP signal is in green to facilitate comparison with FM 4-64 FX. Arrows indicate abnormally enlarged late endosomes typical of the vps4 mutant.