Fig. 7. The localization of TRPL channels in arr2⁵ pupae. Representative cross-sections of single ommatidia from dark-adapted wild type and arr2⁵ null-mutants at 72 hours and 90 hours APF, and post-eclosion, immunostained for TRPL. At 72 hours APF, the rhabdomeres of photoreceptors were visible (phase) with low levels of TRPL staining present in the cell body of photoreceptors. At 90 hours APF, TRPL was localized at the base of the rhabdomeres of wild-type photoreceptors, similar to the adult. At 90 hours APF, TRPL staining in the arr2⁵ pupae appeared in both the rhabdomeres and dispersed in the cell body. This is in contrast to the adult arr2⁵ null-mutant, which displayed TRPL staining in the stalk membranes and at the base of the rhabdomeres. Interestingly, puncta of TRPL staining were present in arr2⁵ pupae 90 hours APF that were not seen in wild type. The corresponding phase-contrast images for the arr2⁵ mutants are shown below (phase). Shown for each genotype and light condition are representative ommatidia from multiple retinal tissue sections; wild-type: five eyes of four flies (72hrs APF), six eyes of five flies (90hrs APF); arr2⁵: four eyes of three flies (72hrs APF), seven eyes of six flies (90hrs APF). For number of eyes and flies used for adult ommatidia, see Figs 1 and 5.