Fig. 6. Ect2 targets RhoA and Citron kinase to the cleavage furrow. (A) HeLa S3 cells were treated for 36 hours with control (GL2) or Ect2 siRNAs and the myc-Ect2_1-333-expressing cell line was induced for 36 hours with tetracycline. After fixation and permeabilization with 10% TCA and Triton X-100, siRNA-treated cells (upper and middle panels) were stained with anti-Ect2 (left, red) and anti-RhoA (middle, green) antibodies. The stable cell line (lower panel) was stained with anti-myc 9E10 (left, green) and anti-RhoA (middle, red) antibodies. Merged images are shown on the right with DNA in blue. (B) Cells were fixed with paraformaldehyde containing Triton X-100 and stained with anti-Citron kinase antibodies. Merged pictures are shown on the right with Ect2 and myc, respectively, in green, Citron kinase in red and DNA in blue. Bars, 10 µm.