JCS -- Nyman-Huttunen et al. 119 (15): 3057 Figure IG2

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Fig. 2. Co-immunoprecipitation of ICAM-5 with ${alpha}$ -actinin. (A) ${alpha}$ -actinin was immunoprecipitated from Paju-ICAM-5 cell lysates with a specific anti- ${alpha}$ -actinin antibody and the precipitates were subjected to SDS-PAGE and immunoblotted with anti-ICAM-5 antibody to detect possible co-immunoprecipitation of ICAM-5. The successful precipitation of ${alpha}$ -actinin was ensured by immunoblotting with anti- ${alpha}$ -actinin antibody (not shown). (B) ICAM-5 was immunoprecipitated from rat brain homogenates and the precipitates were run on SDS-PAGE and immunoblotted for ICAM-5 and after stripping for ${alpha}$ -actinin.