Fig. 4. The distribution of ICAM-5,
-actinin and F-actin in different transfected Paju cell lines. The Paju-neo (A-C,a-c), Paju-ICAM-5 (D-F,d-f), Paju-ICAM-5-TM (G-I,g-i), Paju-ICAM-5-GPI (J-L,j-l), and Paju-ICAM-5-KK/AA cells (M-O,m-o) were double-stained for ICAM-5 (green) and
-actinin (red, left panel) or F-actin (red, right panel) as described in Materials and Methods, and analyzed by confocal microscopy. The regions where the two proteins colocalize appear in yellow. Bars, 10 µm.