Fig. 5. Colocalization of ICAM-5 with -actinin in cultured rat hippocampal neurons. The 7-day-old (A-F) or 14-day-old neurons (G-L) were stained for ICAM-5 (green) and -actinin (red), and analyzed by confocal microscopy. Both ICAM-5 and -actinin showed a punctated expression pattern along the dendrites. The colocalization of the two molecules in apical dendritic filopodia (indicated by arrows, image F) at day 7, and in dendritic spines at day 14 (indicated by arrowheads, image L) are shown under high magnification. Bars, 10 µm.