Fig. 7. Effect of ICAM-5 cytoplasmic peptides on cell morphology. Paju-ICAM-5 (A,C,E) and Paju (B,D,F) cells were incubated with penetratin-coupled ICAM-5_857-861 and ICAM-5-K₈₅₇-K₈₅₈/A-A peptides at 20 µM concentrations. After 2 hours of incubation the cells were washed and bright-field pictures were taken to visualize the effects of the peptides on cell morphology. Bars, 20 µm. The relative percentage of round-shaped Paju-ICAM-5 cells (treated with ICAM-5_857-861 peptide) was 95.2%, and the difference is statistically significant (P<0.001) when compared with the cells treated with ICAM-5-K₈₅₇-K₈₅₈/A-A peptide or without treatment (data not shown).