Fig. 2. Expression of Dok-4 induces neurite outgrowth in TGW cells. (A) Neurite outgrowth of TGW cells transfected with Dok-4. (a) Expression of Dok-4V5 was monitored by western blot analysis. (b) TGW cells stably expressing Dok-4 [TGW(Dok-4)] or parental TGW cells (TGW) were serum-starved and incubated for 48 hours with or without GDNF (50 ng/ml) in the presence or absence of DMSO (0.1%), PD98059 (18 µM), LY294002 (15 µM) or SP600125 (20 µM). (B) Mean of the longest neurite was determined for each culture from measurements of 100 neurons for three different experiments. Each data point represents the mean ± s.e.m. (^*P<0.05).