Fig. 1. Deletion of FIS1, CAF4 or MDV1 influence the relative sizes of the mitochondria-associated Dnm1p-GFP pools. The histogram displays the relative amounts of mitochondria-associated Dnm1p-GFP analyzed on a single-cell basis. 100% equals the whole cellular Dnm1p-GFP pool of the respective cell. For the analysis, fixed cells expressing Dnm1p-GFP and mtDsRed were imaged by using single-molecule-sensitive quantitative confocal fluorescence microscopy. The obtained image data sets were semi-automatically scrutinized. For each strain 80 cells were analyzed.