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Figure 2


Fig. 2. Cytosolic and mitochondria-associated Dnm1p-GFP assemblies have different size distributions in wt, mdv1{Delta}, fis1{Delta} and caf4{Delta} cells. (A) Fluorescence intensity distribution of GFP-VLPs normalized to 1. The major peak corresponds to single GFP-VLPs, whereas the second peak corresponds to two aggregated GFP-VLPs. A single GFP-VLP contains 120 GFP molecules. (B-E) Fluorescence intensity distributions of mitochondria-associated (gray) and cytosolic (black) Dnm1p-GFP assemblies normalized to the mean fluorescence intensity of single GFP-VLPs. For each graph 80 cells expressing Dnm1p-GFP and mtDsRed were imaged and analyzed. Note the differing y-axes values in (E). Insets show representative fluorescence images. Bar, 1 µm.