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Figure 2


Fig. 2. Specificity of the 10E6 and 7D3 monoclonal antibodies for erlin-1. Lipid raft isolates from U937, 3T3-pBabe, 3T3-erlin-1 and 3T3-erlin-2HA cells were subjected to SDS-PAGE in quadruplicate and western blotted using (A) 10E6, (B) 7D3, (C) 12CA5 (anti-HA) or (D) anti-flotillin-1. Arrow in C indicates the undissociated, dimeric form of erlin-2HA. Molecular size markers (kDa) are indicated on the left.