Fig. 3. Epitopes for both 10E6 and 7D3 map to a 25 amino acid region in the C-terminus of erlin-1. (A) Schematic representation of GST-erlin-1 constructs. Numbers indicate bordering amino acid residues of the various regions of erlin-1. Black-filled regions represent the region wherein the 10E6 and 7D3 epitopes lie as deduced by the experiments below. Protein extracts from E. coli expressing GST fusions with erlin-1 were analyzed by western blotting with (B) 10E6, (C) 7D3 or (D) anti-GST.