Fig. 8. Other PHB family members do not localize to the ER. HeLa cells were transfected with pEGFP-N1 containing full-length inserts of prohibitin-1, stomatin-1 isoform a, and flotillin-1 as indicated. Cells were counterstained using antibodies to the ER marker protein calnexin (red). White arrowheads indicate localization to intracellular, vesicular compartments. Bar, 10 µm.