Fig. 9. The extreme N-terminus of each PHB family member is sufficient for its appropriate subcellular targeting. (A) Schematic representations of GFP fusion constructs with the N-terminal fragments of PHB family members. Black-filled regions represent the putative transmembrane domains of the proteins. HeLa cells were transfected with (B) erlin-1, and erlin-2 N-terminal fusions with GFP (as indicated) and counterstained for the transmembrane ER marker protein, calnexin (red), (C) prohibitin 1 N-terminal fusion with GFP and counterstained with MitoTracker CMX-Ros (red), or (D) stomatin 1 isoform a N-terminal fusion with GFP and counterstained with the lipophilic dye SP-DiI₁₈ (red). Bar, 10 µm.