Fig. 5. Expression of Bcl-xL reduces S. cerevisiae acetic-acid-induced ROS production. Co-expression of PKC-, -, - or - differently modulates Bcl-xL effects on ROS production. ROS production by mitochondria was detected by flow cytometry, using MitoTracker Red CM-H₂XRos. Overlay of red fluorescence histograms was obtained with yeast treated with 0 and 300 mM acetic acid. Dark grey fill, cells co-transformed with the empty vectors (pOW4 and YEp51 or pOW4 and YEplac181); light grey line, cells expressing Bcl-xL; black line, cells co-expressing Bcl-xL and PKC-, -, - or -. Cells treated in the absence of acetic acid (0 mM) were used as negative control. Data represent one of two independent experiments.