Fig. 2. Microtubule dynamics during spindle initiation. (A-J) Microtubule dynamics revealed by GFP-AtEB1 microtubule labelling during spindle assembly. Red arrowheads (B) show microtubule plus ends and red arrows (D,E) show microtubule bundles. (K) Fluorescence intensity profile of GFP-AtEB1 along the spindle axis (longitudinal axis extending through poles), corresponding to A,D and H. (L-U) Combined dynamics of microtubule (green) and chromosome (red) during spindle assembly. White arrows and white arrowheads point to microtubule plus ends and the yellow arrow shows the nucleolus. Bar, 5 µm; time is indicated in seconds.