Fig. 7. Dynamics of chromosome movements during anaphase. Green: GFP-AtEB1, and Red: SYTO82-DNA staining (chromosomes inside and mitochondria outside the spindle area). (A-F) Combined microtubular and chromosome dynamics at anaphase (Movie 3 in supplementary material). (G) Kymograph obtained by drawing a line on one of the chromosomes from the anaphase spindle. (H) Phases of poleward motion of chromosome during anaphase. (I) Merged kymograph showing both microtubule growth and chromosome motion. (J) Fluorescence intensity profile of GFP-AtEB1 along the spindle axis (pole to pole axis) during anaphase obtained from lines drawn at three time points (shown in G). The x axis represents pole to pole distance in µm and y axis shows fluorescence intensity in an arbitrary scale. Note that all three panels are at the same fluorescence intensity scale. (K) Speed of individual chromosome motion (represented by each dot) along with its respective position in the spindle. 0 represents a pole-to-pole axis passing through the centre of the spindle equator and left and right sides depict the distance in relation to this spindle axis. The dots show the relative positions of various chromosomes from the spindle axis. Blue dots show the speed of chromosomes during the first (fast) phase of anaphase, whereas green dots show the speed of chromosomes during the second (slow) phase of anaphase. The y axis represents the speed (µm/minute) of individual chromosomes. Bar, 5 µm; time is indicated in seconds.