Fig. 8. Occurrence of microtubule flux in plant spindles. (A-D) Movement of photobleached lines marked on spindle microtubules during metaphase (Movie 4 in supplementary material). (E-H) Movement of photobleached lines marked on spindle microtubules during anaphase (Movie 5 in supplementary material). Panels I and K represent fluorescence intensity profiles projected sequentially on time axes showing translocation of the photobleached area corresponding to images A-D and E-H, respectively. t represents time in seconds and d indicates distance in µm. J represents a difference kymograph of I, showing the gain of fluorescence along the spindle axis immediately after photobleaching, clearly demonstrating microtubule translocation towards the spindle poles. Bar, 5 µm; time is indicated in seconds