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Figure 7


Fig. 7. Organization of matrix-adhesion sites and activation of FAK in crest cells cultured on LN-1 and E8 and E1' fragments. (A-F) Immunofluorescence staining for the ß1-integrin subunit (A-C) and paxillin (D-F) in cells cultured for 24 hours on LN-1 (A,D), E8 (B,E) and E1' (C,F). Arrowheads point at focal contacts, arrows at the periphery of the lamellipodium of the cell. (G) Immunoblotting for phosphorylated FAK on Y397 (upper panel) and total FAK (lower panel) on lysates of cells cultured on LN-1, heat-denatured LN-1 (D-LN1), E8 and E1'. (H) Immunoblotting for phosphorylated FAK on Y397 on lysates of cells cultured on LN-1 and antibodies against {alpha}1ß1. Equivalent amounts of material was loaded in G and H.