Fig. 2. Jun siRNA inhibits growth of murine microvascular endothelial cells. Growth-quiescent bEND-3 cells transfected with 0.02 µM (thin bar) or 0.05 µM (thick bar) of Jun siRNA, siRNAscr, siRNA2, siRNA2scr or mock transfected were incubated in medium containing serum for 48 hours before trypsinisation and quantification of cell numbers using a Coulter counter. The data are representative of at least two independent experiments performed in triplicate. ^*P<0.05 compared with the no siRNA control by Student's t-test.