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Figure 2


Fig. 2. Specificity of the monoclonal DM1A antibody to {alpha}1-tubulin and {alpha}3-tubulin, the constitutively expressed and evolutionary highly conserved {alpha}-tubulin isoforms, and of rabbit anti-{alpha}4-tubulin polyclonal antibody that recognizes only {alpha}4-tubulin. Notice that DM1A does not recognize {alpha}4-tubulin. There is no {alpha}4-tubulin in egg cytoplasm of kavar0/- females. (Dilutions were 500-fold for DM1A and 2000-fold for anti-{alpha}4-tubulin.)