Fig. 3. Localization of 1-tubulin and 3-tubulin as well as 4-tubulin throughout the eleventh cleavage cycle in a wild-type embryo. The constitutively expressed 1-tubulin and 3-tubulin isoforms were detected with the monoclonal DM1A primary and a Texas-Red-labeled fluorescent secondary antibody (merged images). 4-tubulin was detected by an affinity-purified rabbit polyclonal anti-4-tubulin primary antibody and an FITC-labeled fluorescent secondary antibody (merged images). DNA appears in blue (merged images). Arrowheads indicate interpolar microtubules.