Fig. 6. E82K-4-tubulin is incorporated into the microtubules during in vitro tubulin-polymerization. Microtubule formation was induced in egg extracts of +/- (contains one wild-type Tub67C gene) and Kavar^18c/- females. Formed microtubules were pelleted. Egg extracts, supernatant and microtubule-containing pellet were analyzed by SDS PAGE stained with Coomassie Blue and western blot. The 1-tubulin and the 3-tubulin isoforms were detected with monoclonal DM1A primary and a Texas-Red-labeled fluorescent secondary antibody, 4-tubulin by a polyclonal anti-4-tubulin primary and an FITC-labeled fluorescent secondary antibody.