Fig. 8. Ectopically expressed 4-tubulin and E82K-4-tubulin are incorporated into the microtubules in the neuroblast as shown by the encircled spindle apparatus. Ectopic expression of 4-tubulin and E82K-4-tubulin was achieved by driving UAS-Tub67C and UAS-Kavar^18c transgenes with the nervous-system-specific elav-Gal4 driver. 1-tubulin and 3-tubulin (red) were detected with monoclonal DM1A primary and a Texas-Red-labeled fluorescent secondary antibody, 4-tubulin and E82K-4-tubulin (both green) with a polyclonal primary anti-4-tubulin and a FITC-labeled fluorescent secondary antibody; DNA is stained blue.