Fig. 6. Distribution of ER proteins between SER patches and polygonal meshwork ER. (Top row) HeLa TetOff cells expressing GFP-b(5)tail and microinjected with a plasmid coding for G2A b5R were treated with PDMP and immunostained for b5R. Three bottom rows: PDMP-treated cells were immunostained for Sec61ß (second row), CLIMP-63 (third row) or calreticulin (bottom row), with the use of Alexa Fluor 568-conjugated secondary antibodies. The cells were then analysed by confocal microscopy. The arrows in the Sec61ß and CLIMP-63 panels indicate evident SER patches with low content of the two RER markers. Note the tendency of CLIMP-63 to encircle the SER patches. Bars, 10 µm.