JCS -- Sprocati et al. 119 (15): 3249 Figure IG8

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Fig. 8. Sucrose density gradient analysis of PDMP-treated and untreated cells. PNS obtained from total homogenates of induced untreated (A) or PDMP-treated (B) HeLa TetOff cells were centrifuged on sucrose density gradients. An equal aliquot of each fraction was analysed by western blotting for Sec61ß and GFP-b(5)tail, as indicated. The grey line in both panels indicates the percentage of sucrose in each fraction, determined gravimetrically. The arrow and arrowhead below the abscissa indicate the average sucrose concentration at which elements containing GFP-b(5)tail (arrow) and Sec61ß (arrowhead) equilibrate.