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Figure 2


Fig. 2. Interaction between ADAM22 and three 14-3-3 protein family members in yeast two-hybrid reporter plate assays. MaV203 yeast strain containing ADAM22v1 cytoplasmic domain, ADAM22v4 cytoplasmic domain or empty pDBLeu bait vector with pPC86 library clones expressing 14-3-3 protein family members ß, {zeta}, {tau} or vector alone as isolated from a yeast two-hybrid screen. Control A: no interaction strength; control B: weak interaction strength; control C: moderately strong interaction strength; control D: strong interaction strength; control E: very strong interaction strength. Growth on SD/-Leu/-Trp master plates for bait/library plasmid maintenance, SD/-Leu/-Trp/-His/+30 mM3AT for HIS3 reporter expression, SD/-Leu/-Trp/-Ura for URA3 reporter expression, and X-gal assays on nitrocellulose membrane for lacZ reporter expression.