Fig. 7. Palladin co-localizes with Eps8. A7r5 cells were plated on fibronectin-coated coverslips and were either incubated for 2 hours in serum-free media prior to treatment with PDGF (A) or treated with the phorbol ester PDBu (B). Cells were fixed and immunolabeled with polyclonal anti-palladin antibody and monoclonal anti-Eps8 antibody. The two images were merged (overlay) to show the relative localization of palladin (green) and Eps8 (red). Top: low magnification image. Bottom: high magnification image to show detail.