Fig. 3. BNIP-H expression in different parts of the CNS. (A) BNIP-H expression was examined by western blotting with different mouse tissue lysates. A purified polyclonal antibody against human BNIP-H was used. Anti-ß-tubulin antibody was used to demonstrate equal loading. (B-E) BNIP-H expression in different parts of the CNS was examined by immunohistochemistry with mouse brain sections. Strong expression was detected in CA3 of the hippocampus (B), the cerebellar cortex (C), the deep cerebellar nuclei (D) and the pontine nuclei (E). Insets show a higher magnification of the boxed area. CA3, CA3 region of the hippocampus; C, cortex. Bars, 500 µm (B); 100 µm (C-E).