Fig. 5. BNIP-H expression as revealed by ISH histochemistry. BNIP-H expression was examined by ISH histochemistry with rat brain sections as described in the Materials and Methods. Digoxigenin-labelled riboprobe was generated with rat BNIP-H cDNA as template. Coronal sections showed strong staining of the cortex and hippocampus (A), the deep cerebellar nuclei (B), the cerebellar cortex (inset of B) and the spinal cord (C). C, cerebral cortex; ca, ventral horn; cg, central grey matter of the spinal cord; cpo, dorsal horn; GCL, granule cell layer; GD, dentate gyrus; i, interpositus nucleus; l, lateral cerebellar nucleus; m, medial cerebellar nucleus; ML, molecular layer; PCL, Purkinje cell layer. Bars, 2 mm (A,B); 100 µm (inset in B); 200 µm (C).