Fig. 7. Effect of CK2 inhibitors on HIF-1 transcriptional activity in Hep3B cells. Cells were incubated for 16 hours under normoxia or hypoxia in the presence or the absence of different concentrations (µM) of DRB or apigenin (API). (A) Cells were cotransfected with pGL3-6HRE and pRL plasmids. HIF-1 activity is expressed as the ratio of firefly luciferase to Renilla luciferase activity (means ± 1 s.d.; n=3). (B) Real-time PCR analysis of the aldolase mRNA level. Relative aldolase mRNA levels were calculated as the ratio of aldolase expression in hypoxia-incubated cells to these in control normoxic cells, both normalised to -tubulin levels. ^**P<0.01, ^***P<0.001 or NS, not significant vs. normoxia; (^*)P<0.05 or (NS), not significant vs. hypoxia.