Fig. 2. ET-1 stimulates positive inotropy and SCTs in ventricular myocytes. (Ai-iv) Traces depict the effect of continuous superfusion of ventricular myocytes with ET-1 (100 nM). The ET-1 superfusion was initiated just after the trace in i was recorded. Arrows indicate field stimulation. (B) Normalised response (mean ± s.e.m.; n=14 cells) of ventricular myocytes to electrical pacing over a 25-minute period. ^*P<0.05; calculated using Student's t-test). (C) Montages of confocal images collected at 210-millisecond intervals obtained from a single ventricular myocyte loaded with Fluo-4 either (i) before, (ii) 5 minutes after or 12.5 minutes (iii) after the start of superfusion with ET-1. The cell was field-stimulated at the times indicated. Bar, spatial scale of 15 µm.