Fig. 5. Characteristics of SCTs observed during ET-1 superfusion. (A) Average frequency (mean ± s.e.m.; n=14 cells) of SCTs during continuous superfusion of isolated ventricular myocytes with ET-1 (100 nM). (B) Time of occurrence of the SCTs relative to the previous field-stimulation-evoked Ca2+ signal. (C,D) Number of spontaneous Ca2+ sparks recorded using confocal imaging of electrically (C) paced or (D) unpaced cells.