Fig. 4. Cell migration by salivary epithelial cells is not affected by inhibiting cell proliferation. E12 SMG epithelial rudiments were labeled with GFP and Alexa Fluor-647-FN and treated with 0.5 mM hydroxyurea (supplementary material Movie 5). (A) Cells were tracked manually, and 10 representative tracks for each treatment are displayed as 3D rose plots. (B) To measure inhibition of cell proliferation after 24 hours in culture, SMGs were pulse-labeled with BrdU for 3 hours. The mitotic index was calculated as the ratio of total BrdU pixels to total SMG area. Velocity, total distance traveled in 5 hours, displacement and meandering index were calculated as an average of all tracks, and displayed as bar graphs comparing control (black) with hydroxyurea (blue), mean ± s.e.m. Cells were tracked for 3 hours; n=20, averaged from two experiments. Values displayed represent mean ± s.e.m. The Wilcoxon signed-rank test was applied to compare cell tracks of inhibitor-treated SMGs with untreated SMGs, ^*P<0.05 compared with untreated SMGs.